How we can Design Primer? Primer Blast. Primer Specificity. forward and reverse primer, GC and Tm?
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How we can Design Primer? Primer Blast. Primer Specificity. forward and reverse primer, GC and Tm?
Primers are used to amplify ORF in PCR. Primers are of basically two types forward primer and reverse primer. primers start from 5 prime and ends at 3 prime. The primer design is an important step to get an optimal PCR. If you pick up primers without design, the amplification may not work or give you "strange" results, for example if the primer ...
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