Lineweaver Burk Plot Km and Vmax explained
7:26
YouTubeBioScience Class
Lineweaver Burk Plot Km and Vmax explained
This video gives a very simple explanation on how to derive the Lineweaver burk’s plot from the Michaelis-Menten equation.The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y-intercept of ...
1.6K viewsDec 6, 2020
Lineweaver–Burk plot Tutorial
Write the significance of Lineweaver-Burk plot... | Filo
Write the significance of Lineweaver-Burk plot... | Filo
askfilo.com
3 months ago
Use the relationships revealed by a Lineweaver-Burk plot and the table of enzyme performance to calculate the Vmax and KM of the enzyme with no inhibitor, with inhibitor A, and with inhibitor B.1/Vo Slope = KM/VmaxIntercept = -1/KMIntercept = 1/Vmax 01/[S][S] (??)Vo (µmol/min);with no inhibitorVo (µmol/min);with inhibitor AVo (µmol/min);with inhibitor B310.44.12.1514.56.42.91022.511.34.53033.822.66.89040.533.88.1
Use the relationships revealed by a Lineweaver-Burk plot and the table of enzyme performance to calculate the Vmax and KM of the enzyme with no inhibitor, with inhibitor A, and with inhibitor B.1/Vo Slope = KM/VmaxIntercept = -1/KMIntercept = 1/Vmax 01/[S][S] (??)Vo (µmol/min);with no inhibitorVo (µmol/min);with inhibitor AVo (µmol/min);with inhibitor B310.44.12.1514.56.42.91022.511.34.53033.822.66.89040.533.88.1
numerade.com
Sep 17, 2023
Step 5: Estimate Parameters from the Lineweaver-Burk PlotUsin... | Filo
Step 5: Estimate Parameters from the Lineweaver-Burk PlotUsin... | Filo
askfilo.com
5.2K views7 months ago
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